Peer-reviewed veterinary case report
Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2016
- Authors:
- Pearson, James A & Wong, F Susan
- Affiliation:
- Institute of Molecular and Experimental Medicine · United Kingdom
Abstract
MHCI-peptide tetramer staining is an important technique in order to identify antigen-specific T cells within a heterogeneous cell population. The reagents may be used to isolate antigen-specific T cells and can help identify their role in disease. Here, we describe how to make tetramer from peptide:MHC monomers together with a protocol for staining antigen-specific cell populations with advice on generating a complementary antibody phenotyping panel.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26659799/