Identification of novel tumour‐associated antigens in canine mammary gland tumour

Abstract

AbstractCanine mammary gland tumour (MGT) is the most common neoplasm in female dogs and has similar biological characteristics to humanMGT. Spontaneous canineMGTis a more attractive clinical model in oncological research than that of the murine experimental model. Tumour‐associated antigens (TAAs), which are produced in tumour cells, are applied as tumour markers, tumour vaccine antigens and molecular targets of therapeutic drugs. In this study, we have primarily identified 13 differentTAAsof canineMGTby serological immunoscreening ofcDNAexpression library. The results of serological mini‐arrays of identified antigens showed thatCCDC41antigen specially reacted with 35% of sera fromMGT‐dogs and did not react with control sera. We also found thatHSPH1 mRNAexpression levels increased significantly inMGTtissues. These findings will contribute to the development of diagnostic technologies and translational target therapies for dogs.Clinical relevance: HSPH1, which is strongly expressed in the tumour tissue, will be a possible vaccine antigen of canine MGT.