Identification ofandas causative agents of aspergillosis in endangered Okinawa Rails.

Abstract

INTRODUCTION: Aspergillosis is a serious infectious disease in avian species, including endangered birds. However, reports in such species remain scarce. This study focused on the Okinawa Rail (), an endangered flightless bird restricted to the northern forests of Okinawa Island, Japan. METHODS: Air sac tissues and swabs from two deceased Okinawa Rails were cultured on potato dextrose agar. Identification of isolates was confirmed by ITS andsequencing. Antifungal susceptibility was tested using CLSI M38 broth microdilution. Aflatoxin production of isolates was assessed by dichlorvos-ammonia method and quantified via ELISA and LC-MS. Genomic DNA from these isolates was extracted and sequenced using PacBio technology, followed by genome assembly and analysis of antifungal resistance genes and secondary metabolite clusters. RESULTS AND DISCUSSION: We identified and characterizedandas causative agents of aspergillosis in two captive Okinawa Rails. In Case 1, an.isolate showed low susceptibility to voriconazole. It remains unclear whether this low susceptibility was intrinsic or acquired during antifungal treatment. Two phenotypically distinct isolates of.were obtained from the second case. One of them lacked sclerotia and aflatoxin production, suggesting a possible adaptation during infection. This is the first report of.isolation from animal hosts in Japan. Accurate identification and genomic analysis ofisolates provide insights into antifungal resistance and the ecological dynamics of aspergillosis in Okinawa's forest environment, with important implications for wildlife conservation.