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Peer-reviewed veterinary case report

Imaging neural stem cell fate in mouse model of glioma.

Journal:
Current protocols in stem cell biology
Year:
2009
Authors:
Shah, Khalid
Affiliation:
Massachusetts General Hospital · United States

Abstract

This unit describes a protocol for following the fate of stem cells in real time in a mouse model of glioma. Stem cells and tumor cells can be transduced with lentiviral vectors bearing two different luciferases, firefly luciferase (Fluc) and Renilla (Rluc) luciferase, respectively. With the cells labeled in this manner, bioluminescence imaging can be used to study the fate of stem cells in glioma-bearing brains in vivo.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/19306259/