Immunoglobulin Y antibodies against colonization-related genes block the growth and infection of.

Abstract

INTRODUCTION: Immunoglobulin Y (IgY) has emerged as a promising antibody therapy for the eradication of() independent of antibiotics. However, the roles and differences of IgY antibodies targeting various genes againstremain unclear. METHODS: The recombinant antigens of five colonization-related genes -,,,, and- are prepared using a prokaryotic expression system and then subject to immunize laying hens for IgY production. Subsequently, their biological activities are evaluated, including blocking bacterial growth, attenuating infection in GES-1 cells, and eradicatingin gastritis mouse models. RESULTS: These IgY antibodies can recognize the full-length antigens ofand exhibit a direct inhibitory effect on the growth and infection ofwith dose-dependent characteristics. Among these, anti-IgY shows greater antibacterial activity in inhibitinggrowth and preventing adhesion to GES-1 cells. Oral administration of these IgY antibodies for two weeks (20.0 mg·kg·day) achieves a 25% to 37.5% eradication rate ofinfection in mice. Interestingly, combination treatment with these IgY antibodies, based on their different roles, enhances antibacterial benefits and significantly promotes the recovery of gastrointestinal function. CONCLUSION: Our results indicate that IgY antibodies against colonization-related genes can directly block the growth and infection of, and combination treatment with these antibodies offers more advantages in combating.