Peer-reviewed veterinary case report
Anti-Mullerian hormone levels in female dogs with ovarian remnant
By Karakas Alkan, Kubra et al.·Published in Reproduction in domestic animals = Zuchthygiene·2019·Department of Obstetrics and Gynecology·View original on PubMed →
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Original publication title: Immunohistochemical and qPCR determination of the expression and serum level of anti-Müllerian hormone in pre-pubertal, intact and ovarian remnant syndrome detected bitches.
- Species:
- dog
Plain-English summary
A group of female dogs, including some with ovarian remnant syndrome (ORS), were tested for a hormone called anti-Müllerian hormone (AMH) to see if it could help diagnose ORS. The study found that AMH levels were highest in dogs during certain stages of their heat cycle, specifically proestrus and oestrus, and were lower in dogs with ORS compared to those in heat. This suggests that measuring AMH levels could be a useful tool for veterinarians when diagnosing ORS in female dogs.
People also search for: dog ovarian remnant syndrome symptoms · AMH levels in dogs · diagnosing ovarian issues in female dogs
Abstract
The aim of this study was to determine the serum concentrations, ovarian presence and expression of anti-Müllerian hormone (AMH) in pre-pubertal, bitches with signs of ovarian remnant syndrome (ORS) and intact bitches. In addition, we aimed to verify the suitability of serum AMH concentrations for diagnostic purposes in sterilized bitches and/or in suspected cases of ORS in the field of veterinary medicine. For this purpose, 36 healthy female dogs divided into six groups: proestrus, oestrus, dioestrus, anoestrus, pre-pubertal and ORS. Serum AMH concentrations were determined by electrochemiluminescence immunoassay, and ovarian presence and distribution of AMH was confirmed by immunohistochemical and qPCR techniques. According to the results of qPCR, while the expression values of AMH were at the highest concentrations in the proestrus and oestrus, there was a statistically significant decrease in these values at the later stages of the cycle (p < 0.05). According to hormone analysis, the serum AMH values of the ORS group had decreased significantly compared with the proestrus and oestrus (p < 0.05). Although serum AMH levels of ORS group were increased compared with anestrus and pre-pubertal groups, this increase was statistically non-significant (p > 0.05). Immunohistochemically, AMH expression was first observed in the granulosa cells of primordial follicles in folliculogenesis. Expression values were the highest in the proestrous and oestrus groups, but values from bitches in later stages of the cycle were statistically significant decrease in comparison with these groups (p < 0.05). As a result, AMH concentration and expression were found to be higher in proestrus and oestrus than in other periods (p < 0.05). In addition, the measurable level of AMH concentration in bitches with ORS is an indication that it can be used in the diagnosis of ORS.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31050839/