Peer-reviewed veterinary case report
Using human CD1a antibody to diagnose canine mast cell tumors
By Yhee, J-Y et al.·Published in Journal of comparative pathology·2008·Department of Veterinary Pathobiology, South Korea·View original on PubMed →
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Original publication title: Immunohistochemical application of an antibody specific for human CD1a to the diagnosis of canine mast cell tumour.
- Species:
- dog
Plain-English summary
A study found that a special antibody could help veterinarians diagnose canine mast cell tumors (MCTs), especially the more difficult grade III tumors that are poorly differentiated and hard to identify. The antibody was tested on tissue samples from 48 dogs with MCTs and successfully labeled all tumor grades, making it a useful tool for accurate diagnosis. This could improve treatment options and outcomes for dogs with these tumors, as early and correct identification is crucial.
People also search for: dog mast cell tumor diagnosis · canine cancer treatment · grade III mast cell tumor in dogs
Abstract
Canine mast cell tumours (MCTs) may be graded microscopically for prognostic purposes. Grade I (well-differentiated) and grade II (intermediate differentiation) tumours have an abundance of metachromatic granules within the cytoplasm; however, grade III (poorly differentiated) MCTs may be difficult to diagnose as they frequently have fewer discernable granules. Herein we report that a cross-reactive anti-human CD1a monoclonal antibody (clone O10) may be used in immunohistochemistry to identify canine MCTs of all grades. The antibody was applied to tissue sections from 48 canine MCTs of different histological grades. Serial sections from each tumour were stained with toluidine blue and safranin O to compare diagnostic sensitivity. All MCTs were labelled positively by the CD1a antibody, but histochemical staining was often equivocal and identification of mast cells was extremely difficult in some cases. This antibody did not label neoplastic cells in cases of canine histiocytoma, plasmacytoma or amelanotic melanoma; therefore, the reagent may be a valuable marker for the diagnosis of canine MCTs, especially those tumours of histological grade III.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/18602642/