Peer-reviewed veterinary case report
Immunostaining of Lymphocytes in Mouse Atherosclerotic Plaque.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2015
- Authors:
- Gisterå, Anton & Ketelhuth, Daniel F J
- Affiliation:
- Department of Medicine
Abstract
Immunostaining can be defined as a method for identification of constituents in situ, e.g., proteins, using specific antigen-antibody interactions, visualized by color or fluorescence. In experimental research of atherosclerosis, immunostaining is a key method used for the characterization of the cellular composition in the atherosclerotic plaques. This includes the quantification of macrophages, smooth muscle cells, and lymphocytes-especially T lymphocytes. In this chapter we focus on basic protocols for characterization of lymphocytes and/or local T lymphocyte-related antigens in mouse atherosclerotic lesions.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26445787/