Peer-reviewed veterinary case report
Improved multiplex PCR method for the rapid detection of beta-lactamase genes in Escherichia coli of animal origin.
- Journal:
- Diagnostic microbiology and infectious disease
- Year:
- 2006
- Authors:
- Pomba, Constança et al.
- Affiliation:
- Faculty of Veterinary Medicine
Abstract
We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (99%) than A (94%).
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/16678992/