Improvement in nanofat preparation technology: Simple and easy-to-use adipose tissue harvesting with Liporevive.

Abstract

<h4>Background</h4>Autologous adipose tissue is widely used in clinical practice as a favorable material for soft tissue filling. Despite the range of approaches for its processing the main aim is investigating a universal and simple method for processing lipoaspirate to obtain nanofat with optimal histological characteristics and cellular composition.<h4>Methods</h4>Lipoaspirates obtained from the abdominal fat were analyzed. Microfat was obtained by Luer-to-Luer Syringe adapter. Mechanical disaggregation of lipoaspirates were obtained by Liporevive equipped with stainless steel meshes. Using 30 or 50 filtrations through 1.4-, 0.8-, 0.6- and 0.4-mm pore size meshes, nanofat-containing samples were further analyzed for the presence of lipids, endothelial and stromal cells and DNA concentration.<h4>Results</h4>The Liporevive-obtained nanofat samples resembled the tissue structure of the microfat sample and revealed non-damaged parts of tissue filled with intact adipocytes. After using meshes with parameters (input/output/number of times) of 1.4/0.6/30, 1.4/0.4/30, 0.8/0.6/30, 1.4/0.6/50 and 1.4/0.4/50, nanofat samples with DNA concentrations similar to microfat were obtained. Decreasing the mesh's pore sizes to 0.4 mm (output) and increasing the number of filtrations to 50 reduced the content of fat droplets in the nanofat samples, as well as stromal, but not in the endothelial cells.<h4>Conclusion</h4>Mechanical disaggregation of lipoaspirate 30 times with Liporevive, using meshes with a pore diameter of 1.4 mm at the input and 0.6 mm at the output, allows obtaining nanofat comparable to lipoaspirate in DNA concentration, stromal, endothelial and adipose-derived stem cells - key components of regenerative potential. The advantage of Liporevive is obtaining nanofat without additional enzyme treatments.