in a Colony of Zebrafish () Used in a Teaching Laboratory.

Abstract

A small colony of zebrafish () experienced 30% acute mortality within a few days after receipt from a commercial source. A few fish presented with small areas of raised scales or tissue necrosis, primarily near the caudal peduncle.(was identified by real-time PCR of pooled zebrafish and swabs of the pre-filter and fine filter pads, with subsequent sequence analysis..is most commonly associated with an enteric septicemia in catfish species and can have significant economic impact on commercial catfish fisheries. However, several references report naturally occurring.infection of nonictalurid fishes, including zebrafish. Ours is the first report demonstrating the use of environmental sampling to identifyin a zebrafish colony by real-time PCR. Moreover, our report indicates that.is a relevant disease for institutions using zebrafish as research species and emphasizes the importance of carefully considering importation and quarantine practices.