In-depth examination of PrPin Holstein cattle carrying the E211K somatic mutation of the bovine prion protein gene (PRNP).

Abstract

Prion diseases are transmissible spongiform encephalopathies caused by deleterious prion protein (PrP) derived from normal prion protein (PrP), which is encoded by the prion protein gene (PRNP). We performed an in-depth examination to detect PrPby using enzyme immunoassay (EIA), real-time quaking-induced conversion reactions (RT-QuIC) and protein misfolding cyclic amplification (PMCA) in nine brain tissues derived from three Holstein cattle carrying the E211K somatic mutation of the bovine PRNP gene. The EIA, RT-QuIC and PMCA analyses were not able to detect the PrPband in any tested samples. To the best of our knowledge, this report is the first to describe an in-depth examination of PrPin cattle carrying the E211K somatic mutation of the bovine PRNP gene.