Peer-reviewed veterinary case report
Saliva protein changes in dogs with oral tumors
By Sekkarin Ploypetch et al.·Published in BMC Veterinary Research·2020·Biochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, GB·View original on DOAJ →
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Original publication title: In-gel digestion coupled with mass spectrometry (GeLC-MS/MS)-based salivary proteomic profiling of canine oral tumors
- Species:
- dog
Plain-English summary
A study found that dogs with oral tumors, including melanoma and squamous cell carcinoma, had higher levels of certain proteins in their saliva compared to healthy dogs. Specifically, two proteins, PTPN5 and p53, were identified as potential markers for these tumors. This means that testing saliva could help in diagnosing and monitoring oral tumors in dogs. The researchers suggest that these proteins might be useful for understanding how the tumors respond to treatment.
People also search for: dog oral tumor symptoms · canine melanoma saliva test · dog squamous cell carcinoma diagnosis
Abstract
Abstract Background Various types of oral tumors, either benign or malignant, are commonly found in dogs. Since saliva directly contacts the tumors and saliva collection is non-invasive, easily accessible and cost effective, salivary biomarkers are practical to be used for the diagnosis and/or prognosis of these diseases. However, there is limited knowledge of protein expression in saliva for canine oral tumors. The present study aimed to investigate novel biomarkers from the salivary proteome of dogs with early- and late-stage oral melanoma (EOM and LOM, respectively), oral squamous cell carcinoma (OSCC), benign oral tumors (BN), and periodontitis and healthy controls (CP), using an in-gel digestion coupled with mass spectrometry (GeLC-MS/MS). The relationships between protein candidates and chemotherapy drugs were explored and the expression of potential biomarkers in saliva and tissues was verified by western blot analysis. Results For saliva samples, increased expression of protein tyrosine phosphatase non-receptor type 5 (PTPN5) was shown in all tumor groups compared with the CP group. Marked expression of PTPN5 was also observed in LOM and OSCC compared with that in BN and EOM. In addition, tumor protein p53 (p53), which appeared in the PTPN5–drug interactions, was exhibited to be expressed in all tumor groups compared with that in the CP group. For tissue samples, increased expression of p53 was shown in LOM compared with the control group. Conclusion PTPN5 and p53 were proposed to be potential salivary biomarkers of canine oral tumors.
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Search related cases →Original publication on DOAJ: https://doi.org/10.1186/s12917-020-02550-w