Peer-reviewed veterinary case report
Antimicrobial gel tested on dog ear infections with Pseudomonas
By Ghibaudo, Giovanni et al.·Published in Veterinary dermatology·2016·Clinica Veterinaria Malpensa, Italy·View original on PubMed →
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Original publication title: In vitro antimicrobial activity of a gel containing antimicrobial peptide AMP2041, chlorhexidine digluconate and Tris-EDTA on clinical isolates of Pseudomonas aeruginosa from canine otitis.
- Species:
- dog
Plain-English summary
A group of dogs with ear infections caused by a tough-to-treat bacteria called Pseudomonas aeruginosa were tested with a special gel containing antimicrobial ingredients. This gel showed strong effectiveness, killing the bacteria quickly—within 30 seconds for all strains tested. Even at lower concentrations, it maintained its ability to eliminate the bacteria for up to 48 hours. This suggests that the gel could be a promising treatment option for dogs suffering from severe ear infections.
People also search for: dog ear infection treatment · Pseudomonas aeruginosa in dogs · antimicrobial gel for dog otitis
Abstract
BACKGROUND: Pseudomonas aeruginosa (PA) may cause suppurative otitis externa with severe inflammation and ulceration in dogs. Multidrug resistance is commonly reported for this organism, creating a difficult therapeutic challenge. OBJECTIVE: The aim of this study was to evaluate the in vitro antimicrobial activity of a gel containing 0.5 μg/mL of antimicrobial peptide AMP2041, 0.07% chlorhexidine digluconate (CLX), 0.4% Tris and 0.1% EDTA on 30 clinical isolates of PA from canine otitis externa. MATERIALS AND METHODS: Antimicrobial activity was evaluated through minimal bactericidal concentration (MBC). Standardized bacterial suspensions were incubated with different concentrations of the gel at 37°C for 30 min and plated for colony forming unit (CFU) counts. Time-to-kill kinetics were evaluated with the undiluted product and at MBC for each PA strain at 30 s, 1, 5, 10, 15, 30 min, 24 and 48 h. RESULTS: The MBC was 1:64 for two of 30 strains, 1:128 for 15 of 30 strains and 1:256 for 13 of 30 strains. The geometric mean was 1:165, equivalent to a concentration of 0.003 μg/mL AMP2041 + 0.0004% CLX + 0.0024%Tris + 0.0006% EDTA. Time-to-kill assays with the undiluted product showed complete bactericidal effect within 30 s for all isolates, whereas at the MBC this effect was reached within 5 min for 20 of 30 isolates and within 30 min for all isolates. Bactericidal activity was maintained after 48 h for all isolates. CONCLUSION: This gel has shown rapid, complete and long-lasting activity against a panel of 30 PA isolates from cases of canine otitis externa.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/27545352/