In vivo bioluminescence imaging of labile iron pools in a murine model of sepsis with a highly selective probe.

Abstract

Iron plays an essential role in biological system. An approach for in vivo imaging of this metal ion is needed to investigate its complex contributions to physiological and pathological processes. Herein, we present a bioluminescent probe FP-1 as a powerful tool for targeting Fedetection in vitro and in vivo. The turn-on sensing scheme is based on the caged strategy of luciferin-luciferase system. FP-1 not only can detect accumulations of exogenous Fein living animal, but also has the capability of monitoring labile endogenous Felevels in animal model of sepsis. Implementation of this technique provides a valuable opportunity for understanding underlying mechanisms of Fein biological processes and disease states.