Peer-reviewed veterinary case report
In Vivo Clonal Analysis of Cardiomyocytes.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2021
- Authors:
- Nguyen, Ngoc B et al.
- Affiliation:
- Department of Internal Medicine · United States
Abstract
The development of the CreER/LoxP system has enabled temporal control and cell type specificity of gene activation or repression. A common application of this system involves lineage tracing and examining the proliferative capacity of cells of interest through clonal analysis. Here, we describe a method of performing 2- and 3-dimensional clonal analysis of cardiomyocytes (CMs) using the Rainbow reporter mouse model. We outline the process of using the Cell Counter plug-in tool in ImageJ to quantify the number of clones as well as the number of cells within each clone. For 3-dimensional analysis, we describe the tissue clearing technique, CLARITY, in conjunction with light-sheet imaging to obtain digital slices of the whole heart that can be reconstructed and clone volumes quantified using the Imaris software.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/32857378/