Peer-reviewed veterinary case report
Bone marrow cells with beta-TCP used to fix dog eye socket bone
By Zhou, Huifang et al.·Published in Investigative ophthalmology & visual science·2011·Department of Ophthalmology, China·View original on PubMed →
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Original publication title: In vivo efficacy of bone marrow stromal cells coated with beta-tricalcium phosphate for the reconstruction of orbital defects in canines.
- Species:
- dog
Plain-English summary
A dog with a significant injury to its eye socket was treated using a special technique involving its own bone marrow cells and a biodegradable material. After creating a 25-mm defect in the orbital rim, the dog's bone marrow cells were cultured and then implanted onto a scaffold made of beta-tricalcium phosphate. Twelve weeks later, scans showed that the area had healed well, with good bone density and structure, while other methods did not achieve the same level of repair. This approach proved effective for repairing serious bone defects in dogs.
People also search for: dog eye socket injury treatment · canine orbital rim repair · bone marrow cells for dog injury · beta-tricalcium phosphate in dogs
Abstract
PURPOSE: To repair the segmental orbital rim defects of dogs with three-dimensional (3D) tissue-engineered constructs derived from culturing autogenous bone marrow stromal cells (BMSCs) on β-tricalcium phosphate (β-TCP) scaffolds. METHODS: A 25-mm segmental defect on the canine inferior orbital rim was created. BMSCs were isolated and osteogenically induced in vitro, then were seeded onto 3D β-TCP scaffolds and implanted to repair the orbital defects after 5 days of cultivation. The group of noninduced BMSC/β-TCP, β-TCP alone, and the normal inferior orbital rim were set as controls. The orbits of all groups had spiral computed tomography (CT) scans 1, 4, 8, and 12 weeks after surgery. Gross examination, bone density, microCT, and histologic measurements were performed 12 weeks after surgery. The results were analyzed to evaluate the extent of bone repair. RESULTS: Twelve weeks after surgery, CT examination revealed good inferior orbital rim recovery in the induced BMSC/β-TCP group, and the bone density was 0.30 ± 0.03 g/cm(2) with no dominant variance, compared with the normal control (P > 0.05). MicroCT and histologic examination confirmed that the implantations led to good repair of the defects. Pore-like spongy bone surrounded the implants through the section plane, with some residue remaining in the center. In contrast, the noninduced BMSC/β-TCP implants were not fully repaired, and nonunion was evident. The bony density for this group was 0.23 ± 0.07 g/cm(2), which was significantly lower than that of the control group (P < 0.05). The β-TCP group was largely held by fibrous tissues. CONCLUSIONS: Engineered bone from induced BMSCs and 3D biodegradable β-TCP can efficiently repair critical-sized segmental orbital defects in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/21087968/