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Peer-reviewed veterinary case report

In Vivo Mapping and Quantification of Creatine Using Chemical Exchange Saturation Transfer Imaging in Rat Models of Epileptic Seizure.

Journal:
Molecular imaging and biology
Year:
2019
Authors:
Lee, Dong-Hoon et al.
Affiliation:
Faculty of Health Sciences and Brain & Mind Centre · Australia
Species:
rodent

Abstract

PURPOSE: To evaluate signal changes in the hippocampus of epileptic seizure rat models, based on quantified creatine chemical exchange saturation transfer (CrCEST) signals. PROCEDURES: CEST data andH magnetic resonance spectroscopy (H MRS) data were obtained for the two imaging groups: control (CTRL) and epileptic seizure-induced (ES; via kainic acid [KA] injection) groups. CrCEST signals in the hippocampal regions were quantitatively evaluated; correlations between CrCEST signals and phosphocreatine (PCr) and total creatine (tCr; PCr&#x2009;+&#x2009;Cr) concentrations, derived from the analysis ofH MRS data, were investigated as a function of time changes (before KA injection, 3 and 5&#xa0;h after KA injection). RESULTS: Measured CrCEST signals were exhibited significant differences between before and after KA injection in the ES group. At each time point, CrCEST signals showed significant correlations with PCr concentration (all |r|&#x2009;>&#x2009;0.59; all P&#x2009;<&#x2009;0.05); no significant correlations were found between CrCEST signals and tCr concentrations (all |r|&#x2009;<&#x2009;0.22; all P&#x2009;>&#x2009;0.05). CONCLUSIONS: CrCEST can adequately detect changes in the concentration of Cr as a result of energy metabolism, and may serve as a potentially useful tool for diagnosis and assessment of prognosis in epilepsy.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/29951847/