Peer-reviewed veterinary case report
In-yeast reconstruction of the African swine fever virus genome isolated from clinical samples.
- Journal:
- STAR protocols
- Year:
- 2021
- Authors:
- Labroussaa, Fabien et al.
- Affiliation:
- Institute of Veterinary Bacteriology
Abstract
This protocol describes a synthetic genomics pipeline to clone and engineer the entire 190-kbp genome of the African swine fever virus (ASFV) genotype II in yeast using transformation-associated recombination cloning. The viral genome was cloned using DNA directly extracted from a clinical sample. In addition, the precise deletion of a non-essential gene and its replacement by a synthetic reporter gene cassette are presented. This protocol is applicable to other ASFV genotypes and other large DNA viruses.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/34527959/