Peer-reviewed veterinary case report
Inducing Pluripotency in Cattle.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2015
- Authors:
- Malaver-Ortega, Luis F et al.
- Affiliation:
- Monash Institute for Medical Research · Australia
Abstract
Nuclear reprogramming technologies in general and induced pluripotent stem cells (iPSCs) in particular have opened the door to a vast number of practical applications in regenerative medicine and biotechnology. It also represents a possible alternative to the still evasive achievement of embryonic stem cells (ESCs) isolation from refractory species such as Bos. taurus. Herein, we described a protocol for bovine iPSCs (biPSCs) generation and characterization. The protocol is based on the overexpression of the exogenous transcription factors NANOG, OCT4, SOX2, KLF4 and c-MYC, using a pantropic retroviral system.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26621589/