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Peer-reviewed veterinary case report

Infant Mouse Model of Vibrio cholerae Infection and Colonization.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2018
Authors:
Matson, Jyl S
Affiliation:
Department of Medical Microbiology and Immunology · United States

Abstract

Cholera is an epidemic diarrheal disease caused by Vibrio cholerae that continues to cause significant morbidity and mortality in many parts of the world. Several different animal models have been used by scientists over the years to study the pathogenesis of cholera. However, the most commonly used is the infant (suckling) mouse model, which has been found to replicate important aspects of human intestinal colonization. Here we present a detailed protocol for using the infant mouse model to assess the colonization of V. cholerae strains using a competition assay.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/30047061/