Infection of Adipose Tissues.

Abstract

Adipose tissues were shown to hostwhich is persisting inside mature adipocytes. It remains unknown whether this holds true for, a rare representative of thecomplex responsible for lymphatic and pulmonary tuberculosis. Here, we infected primary murine white and brown pre-adipocytes and murine 3T3-L1 pre-adipocytes and mature adipocytes withandas a positive control. Both mycobacteria were able to infect 18-22% of challenged primary murine pre-adipocytes; and to replicate within these cells during a 7-day experiment with the intracellular inoculums being significantly higher in brown than in white pre-adipocytes for(= 0.02) and(= 0.03). Furtherinfection of 3T3-L1 mature adipocytes yielded 9% of infected cells byand 17% of infected cells by(= 0.001). Interestingly,replicated and accumulated intra-cytosolic lipid inclusions within mature adipocytes over a 12-day experiment; whilestopped replicating at day 3 post-infection. These results indicate that brown pre-adipocytes could be one of the potential targets forcomplex mycobacteria; and illustrate differential outcome ofcomplex mycobacteria into adipose tissues. While white adipose tissue is an unlikely sanctuary for, it is still an open question whetherandcould persist in brown adipose tissues.