Infection ofPrimary Midgut Cell Culture Is Dependent on Fucosylated Glycans.

Abstract

Tick midgut is the primary infection site required by tick-borne pathogens to initiate their development for transmission. Despite the biological significance of this organ, cell cultures derived exclusively from tick midgut tissues are unavailable and protocols for generating primary midgut cell cultures have not been described. To study the mechanism of-tick cell interactions, we successfully developed anprimary midgut cell culture system. Midgut cells were maintained for up to 120 days. We demonstrated the infection ofmidgut cells by using anmutant with continued replication for up to 10 days post-infection.infection of midgut cells regulated the differential expression of tick α-(1,3)-fucosyltransferases A1 and A2. Silencing of α-(1,3)-fucosyltransferase A2 in uninfected midgut cells reduced the display of fucosylated glycans and significantly lowered the susceptibility of midgut cells toinfection, suggesting that the pathogen utilized core α-(1,3)-fucose of N-glycans to infect tick midgut cells. This is the first report usingprimarymidgut cells to study-tick cell interactions at the molecular level. The primary midgut cell culture system will further facilitate the investigation of tick-pathogen interactions, leading to the development of novel intervention strategies for tick-borne diseases.