Influence of Cushion Plant <i>Androsace tapete</i> on Nitrogen Uptake Strategies of Associated Alpine Plants.

Abstract

In alpine ecosystems, plant growth is often constrained by multiple environmental factors, especially the infertile soils with lower temperature that decelerate the rate of nutrient turnover, thus leading to a diminished availability of nutrients in the soil, notably nitrogen (N), and its different forms, which is a pivotal factor for limiting plant growth and species coexistence in these alpine areas. <i>Androsace tapete</i> (<i>A. tapete</i>) is an endemic species and the most widely distributed cushion plant on the Qinghai-Tibet Plateau (QTP). Its positive interactions can facilitate other associated plants to deal with severe environmental conditions in the alpine grassland ecosystem. The change in soil nutrient availability is one of the main positive interactions, but little is known about how <i>A. tapete</i> changes soil nutrient availability and affects the N uptake pattern of associated plants. This study investigated the N utilization patterns of three associated plant species -<i>Carex atrofusca</i> (<i>C. atrofusca</i>), <i>Cyananthus incanus</i> (<i>C. incanus</i>), and <i>Potentilla saundersiana</i> (<i>P. saundersiana</i>)- growing inside the cushion area <i>A. tapete</i> (CA) and the ambient grassland without cushion plants (CK), using a ¹⁵N labeling method to clarify the effect of <i>A. tapete</i> on the N uptake strategies with NH₄⁺, NO₃^-, and organic N of its associated species. The results showed the following: (1) compared to CK, the soil total C, total N, and available NH₄⁺ contents under the <i>A. tapete</i> showed a significant 47.82%, 40.96%, and 47.33% increase, respectively; (2) <i>A. tapete</i> showed a stronger preference for NH₄⁺ (>80%), whereas the associated species in CK exhibited a more balanced uptake, deriving 39.29-55.59% of N from NO₃^-, 25.72-44.00% from NH₄⁺, and 16.15-18.69% from glycine. (3) The three associated plants possessing <i>A. tapete</i> significantly reduced their uptake of glycine by 9.76%, 12.55%, and 7.15%, respectively, while the absorption of NH₄⁺ by <i>C. atrofusca</i> and <i>C. incanus</i> increased by 18.46% and 36.11%; meanwhile, NO₃^- uptake decreased by 8.70% in <i>C. atrofusca</i> and 23.55% in <i>C. incanus</i>. These findings indicated that the <i>A. tapete</i> can change the N uptake pattern of the associated plants growing inside the cushion body, such as enhancing the absorption of inorganic N and decreasing the organic N. This adaptive strategy of the associated plants with cushion plant enables them to counteract the N-limited conditions prevalent in alpine environments, and, as a consequence, facilitates their growth and promotes local plant community diversity in the alpine environment.