Influence of dental materials on cells of the equine periodontium.

Abstract

BACKGROUND: Therapy for equine periodontal disease can include filling of the periodontal pockets and widened interproximal spaces. Recommended dental materials are generally adopted from human dentistry. OBJECTIVES: To evaluate the biocompatibility of dental materials for equine periodontal fillings in vitro. STUDY DESIGN: In vitro experiments. METHODS: Four different dental materials (PeriCare, Provicol, Calxyland Honigum) were tested on equine periodontal fibroblasts. Possible cytotoxic effects were assessed microscopically and by MTT assay, and the expression of inflammatory marker genes was measured by qRT-PCR. RESULTS: PeriCareand Provicolhad no effects on the cells, whereas Honigum and Calxylwere associated with severe cytotoxic effects. MAIN LIMITATIONS: The results of this in vitro study need to be confirmed by clinical studies. CONCLUSIONS: Before adapting dental materials from human dentistry, it is crucial to initially test them in a specific equine model.