Peer-reviewed veterinary case report
Inhibition of hepatitis C virus-transfected cholangiocarcinoma by antisense oligodeoxynucleotide in nude mice.
- Journal:
- Hepatobiliary & pancreatic diseases international : HBPD INT
- Year:
- 2004
- Authors:
- Liu, Xiao-Fang et al.
- Affiliation:
- Department of Hepatobiliary Surgery · China
- Species:
- rodent
Abstract
BACKGROUND: The inhibitory effect of antisense oligodeoxynucleotide (asODN) on the replication and expression of hepatitis C virus (HCV) in vitro is not well elucidated. This study was to assess the effect of asODN on HCV in cholangiocarcinoma. METHODS: The QBC939 cells transfected by a recombinant HCV containing HCV core gene cloned in vector of PBK-CMV (PBK-HCVC) were treated by 14-mers phosphorothioate ODN complementary to the HCV core genomic region. The variation of HCVmRNA level was detected by RT-PCR. Moreover, the inhibitory effect of asODN was observed in nude mice. RESULTS: HCVmRNA was detected in transfected-QBC939 cells. The 14-mers complementary phosphorothioate ODN showed effective inhibition on HCVmRNA and unexpression HCVmRNA at 6 micromol/L. The tumorigenicity of the transfected-QBC939 cells incubated with asODN in nude mice was greatly inhibited. CONCLUSION: The results suggest a potential therapy of asODN for HCV infected cholangiocarcinoma.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/14969852/