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Peer-reviewed veterinary case report

Interference of circulating endogenous antibodies on the Dimension® DGNA digoxin immunoassay: elimination with a heterophilic blocking reagent.

Journal:
Clinical biochemistry
Year:
2010
Authors:
Hermida-Cadahía, Esperanza F et al.
Affiliation:
Hospital Cl&#xed · Spain

Plain-English summary

This study looked at how certain antibodies in the blood can interfere with a test that measures digoxin, a medication often used for heart issues. In one older person who had been around cats, the test showed a slightly higher level of digoxin than it should have, which was fixed by using a special treatment on the blood sample. Another type of test, called the Architect immunoassay, was not affected by these antibodies. Overall, the treatment successfully eliminated the interference in the digoxin test.

Abstract

Only two cases have been previously described about circulating endogenous antibodies interference on the immunochemical determination of digoxin. In an elderly patient with history of social cat handling was observed a moderate positive interference (about 0.8 ng/mL) on the Dimension® DGNA digoxin immunoassay (capture rabbit antibody), which was eliminated treating the serum samples with a heterophilic blocking reagent. The Architect® immunoassay was not affected.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/20875811/