Isolated hepatocytes stimulation activity on toxic liver failure in experimental study.

Abstract

Finding of affective treatment methods of acute liver failure (alf), consists one of difficult solving problem in modern hepatology. To day improving liver transplantation methods for the stimulation of regeneration process. Transplantation of liver allogenic isolated hepatocytes in A.L.F.treatment, promotes regeneration morphological capabilities of toxically damaged liver. We used combined enzymatic-mechanical method modified by Korukhov (1983), which let to take up 50-60% of cells from liver mass, 80% from them maintained their morphological structure and functional activity. Experiments were performed on 45 white laboratory rats. Animals were divided into 3 groups. I group of animals (n=20) was the control group, with acute liver failure without treatment, in this group, lethal outcome was 100% in the I group, after CCL4 administration on 5 and 7 days. II group (n=20) included animals who had ALF model and which provides treatment with isolated hepatocytes transplantation. III group of animals were served as donors for isolated hepatocytes uptake. 90% of animals from II group were alive after treatment during 20 days and then their condition was satisfactory. Biochemical and morphological investigation proved that our treatment method induces toxically damaged liver function restoration.