Peer-reviewed veterinary case report
Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC.
- Journal:
- STAR protocols
- Year:
- 2022
- Authors:
- Alam, Aftab et al.
- Affiliation:
- Department of Immunology · United States
Abstract
Innate lymphoid cells 2 (ILC2) play a significant role in the tumorigenesis of pancreatic ductal adenocarcinoma (PDAC). An important aspect of ILC2-mediated tumorigenesis is the expansion of the resident ILC2 and simultaneous recruitment of the peripheral ILC2. Here, we describe a protocol for isolation, enrichment, and DiD labeling of ILC2 fortracking of ILC2s in the mouse. Further, we describe steps for the adoptive transfer of ILC2 to a recipient mouse model of PDAC. For complete details on the use and execution of this protocol, please refer to Alam et al. (2022).
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/35874471/