Isolation and characterization of a lytic bacteriophage against Staphylococcus aureus.

Abstract

() is one of major pathogens in animal production.-induced mastitis poses a serious threat to animal health and leads to significant economic losses. The objective of this study was to isolate and characterize bacteriophage(s) specific toand assess its (their) in vitro antibacterial activity. A strain ofbacteriophage, designated JXAU-SA-P1, was isolated from sewage in a commercial pig farm. Morphological analysis revealed that JXAU-SA-P1 belongs to theclass. The genome of JXAU-SA-P1 is 134.23 kb in length with a GC content of 29.99%, and no virulence or antibiotic resistance genes were identified. JXAU-SA-P1 exhibited a low optimal multiplicity of infection (0.001), a high burst size (300 PFU/cell), and significant antibacterial activity. The bacteriophage showed stable activity below 50 °C and within a pH range of 2.0–12.0. JXAU-SA-P1 effectively inhibited the growth ofin milk at both 4 °C and 25 °C. Furthermore, it demonstrated substantial biofilm prevention and removal properties. In conclusion, these findings indicate that JXAU-SA-P1 is a potential antibacterial agent for the control of-induced mastitis.