Peer-reviewed veterinary case report
Isolation and genotyping of Toxoplasma gondii from free-range ducks in Malaysia.
- Journal:
- Avian diseases
- Year:
- 2013
- Authors:
- Puvanesuaran, Vignesh R et al.
- Affiliation:
- Institute for Research in Molecular Medicine (INFORMM)
- Species:
- rodent
Abstract
Toxoplasma gondii is a parasitic protozoan that infects nearly one-third of humans. The present study was performed to isolate and genotype T. gondii from free-range ducks in Malaysia. Sera, heads, and hearts from 205 ducks were obtained from four states in Peninsular Malaysia, and 30 (14.63%) sera were found to be seropositive when assayed with the modified agglutination test (MAT > or = 1:6). All the positive samples were inoculated into mice, and T. gondii was successfully isolated from four individual duck samples (1.95%), which were initially found to be strongly seropositive (MAT > or = 1:24). The isolates were subjected to PCR-RFLP analysis, and two T. gondii strains were identified: type I and type II. This is the first reported study on the genetic characterization of T. gondii isolates from free-range farm animals in Southeast Asia.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23678741/