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Peer-reviewed veterinary case report

Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca).

Journal:
Journal of veterinary science
Year:
2009
Authors:
Gao, Feng-Shan et al.
Affiliation:
Department of Biochemistry and Molecular Biology · China
Species:
dog

Abstract

Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/19687628/