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Peer-reviewed veterinary case report

Isolation and molecular characterization of Brucella melitensis from seropositive goats in Peninsula Malaysia.

Journal:
Tropical biomedicine
Year:
2012
Authors:
Bamaiyi, P H et al.
Affiliation:
Faculty of Veterinary Medicine

Abstract

A study was carried out to isolate Brucella melitensis using established bacteriological and PCR techniques in Brucella seropositive goats in farms in Selangor, Negeri Sembilan, Melaka and Pulau Pinang. Brucella melitensis was isolated from 7 of 134 reactors with the highest isolation from the vaginal swabs (57.14%) followed by the spleen (28.57%), uterine fluid (14.29%). No Brucella was isolated from the lymph nodes. PCR confirmed all the seven isolates as B. melitensis and isolates were phylogenetically related to other isolates from India, Iran, and Israel but most closely related to isolates from Singapore.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/23202595/