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Peer-reviewed veterinary case report

Isolation of Moraxella bovoculi from racehorses with keratoconjunctivitis.

Journal:
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Year:
2014
Authors:
Liu, Huimin et al.
Affiliation:
Institute of Zoology · China

Plain-English summary

In a study from China in 2013, researchers found a bacteria called Moraxella bovoculi in the eye fluid of nine racehorses suffering from an eye infection known as keratoconjunctivitis. All the samples showed that this bacteria was capable of breaking down red blood cells and had specific genetic markers that confirmed its identity. The tests indicated that the bacteria were closely related to other known strains of Moraxella bovoculi. This research helps to understand the causes of eye infections in racehorses, but it does not provide information on treatment outcomes.

Abstract

Moraxella bovoculi was isolated and identified in ocular fluid samples collected from 9 racehorses with infectious keratoconjunctivitis in China in 2013. All 9 M. bovoculi isolates were hemolytic, Gram-negative diplococci that were phenylalanine deaminase positive. The sequence of the 16S ribosomal DNA (rDNA) gene of the isolates matched the 16S rDNA sequence of M. bovoculi. Amplification of the 16S-23S intergenic spacer region followed by AfaI digestion produced a 600-base pair product, a result characteristic of M. bovoculi isolates. The phylogenetic analysis based on the 16S rDNA sequence confirmed the strain isolated in the current study had genetic homology with M. bovoculi.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/24903634/