Peer-reviewed veterinary case report
Isolation of Porcine Bone Marrow Cells and Generation of Recombinant African Swine Fever Viruses.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2022
- Authors:
- Rathakrishnan, Anusyah et al.
- Affiliation:
- The Pirbright Institute · United Kingdom
Abstract
Genetic manipulation of ASFV has been increasingly used not only for the development of live attenuated vaccines but also as an indispensable tool to further our understanding of the virus-host interactions. Here we present methods for isolation of porcine bone marrow cells and purification of recombinant ASFV using both chromogenic and fluorescent reporters. We also describe in detail a newly developed method to purify genetically modified ASFV using fluorescence-activated cell sorting (FACS).
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/35575887/