Peer-reviewed veterinary case report
Two new recombinant canine coronavirus strains found in puppies
By Ntafis, V et al.·Published in Veterinary microbiology·2011·Department of Anatomy and Physiology of Farm Animals·View original on PubMed →
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Original publication title: Isolation, tissue distribution and molecular characterization of two recombinant canine coronavirus strains.
- Species:
- dog
Plain-English summary
Two puppies died after showing gastrointestinal symptoms, which included vomiting and diarrhea. Testing revealed they were infected with a new strain of canine coronavirus (CCoV-IIb) along with another virus called canine parvovirus (CPV-2). The researchers found that this new strain was present not only in the puppies' feces but also in their internal organs, suggesting it could spread more easily in dogs already infected with CPV-2. This study highlights the importance of monitoring for different strains of canine coronavirus, especially in puppies with severe gastrointestinal issues.
People also search for: puppy vomiting diarrhea · canine coronavirus symptoms · dog parvovirus treatment
Abstract
Canine coronavirus (CCoV) is an enveloped RNA virus, responsible for gastrointestinal infection in dogs. To date, two different CCoV genotypes have been recognized, CCoV type I and CCoV type II. Recently, CCoV type II strains of potential recombinant origin with transmissible gastroenteritis virus (TGEV) were detected and characterized as a new subtype (CCoV-IIb) of canine coronavirus, in order to be differentiated from the "classical" CCoV type II strains (CCoV-IIa). In the present study, two CCoV-IIb strains were detected in the faeces and internal organs of two puppies, which died after presenting gastrointestinal symptoms. Mixed infection of both subtypes (CCoV-IIa/IIb) was detected in the faeces, while only CCoV-IIb was detected in the organs. Puppies were also infected by canine parvovirus type 2 (CPV-2). Both CCoV-IIb strains were isolated on cell cultures and subjected to sequence analysis and phylogeny. By means of RT-PCR and real time RT-PCR assays, tissue distribution and quantitation of viral loads took place. These cases represent the first description of tissue distribution and quantitation of CCoV-IIb strains, detected in the organs. The detection of CCoV-IIa strains, which is restricted to the faeces, suggests that CCoV-IIb strains may have an advantage in disseminating throughout a dog with CPV-2 coinfection, in contrast to common enteric CCoV-IIa strains.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/21481551/