ITGB1b-Deficient Rare Minnows Delay Grass Carp Reovirus (GCRV) Entry and Attenuate GCRV-Triggered Apoptosis.

Abstract

Integrin β-1 (ITGB1) is a transmembrane protein belonging to the integrin family and it plays an important role in viral entry. In this study, thegene of the rare minnow,, was cloned and analyzed. To investigate the possible role ofon grass carp reovirus (GCRV) infection, we generated an ITGB1b-deficient rare minnow (ITGB1b) using the CRISPR/Cas9 system. Following stimulation with GCRV, the survival time of the -ITGB1brare minnows was extended in comparison to the wild-type minnows. Moreover, the relative copy number of GCRV and the level of clathrin-mediated endocytosis-associated and apoptosis-related gene expression in the ITGB1brare minnows was significantly lower than that of the wild-type minnows. These results suggested that the absence ofreduced viral entry efficiency and the expression of apoptosis-related genes. Moreover, the data suggested thatplayed an important role in mediating the entry of viruses into the cells via clathrin. Therefore, these findings provide novel insight into the function ofin the process of GCRV infection.