Peer-reviewed veterinary case report
Ki-67 and CD3 markers help diagnose chronic gut inflammation in dogs
By Karlovits, Sonja et al.·Published in Journal of veterinary internal medicine·2020·Department for Companion Animals and Horses·View original on PubMed →
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Original publication title: Ki-67/CD3 ratio in the diagnosis of chronic inflammatory enteropathy in dogs.
- Species:
- dog
Plain-English summary
A group of dogs with chronic inflammatory enteropathy (CIE), which causes symptoms like vomiting and diarrhea, were studied to see if a specific test could help diagnose the condition. Researchers found that dogs with CIE had higher levels of certain immune cells compared to healthy dogs. This test, which measures the ratio of Ki-67 (a marker for cell growth) to CD3 (a marker for T cells), was linked to the severity of the dogs' symptoms. This suggests that the Ki-67/CD3 ratio could be a helpful tool for veterinarians in diagnosing CIE in dogs.
People also search for: dog vomiting diarrhea treatment · chronic inflammatory enteropathy in dogs · Ki-67 test for dog health
Abstract
BACKGROUND: T cells play a key role in the pathogenesis of chronic inflammatory enteropathy (CIE) in dogs. Cluster of differentiation 3 (CD3) antigen serves as a marker for T cells. In human medicine, Ki-67 is an indicator for cell growth but there are only a few studies in dogs with CIE. OBJECTIVE: To investigate Ki-67 in relation to T cells as a marker for CIE in dogs. ANIMALS: Eleven dogs with CIE and 6 healthy beagle controls (CO). METHODS: Retrospective case-control study. Dogs were clinically assessed by the Canine Chronic Enteropathy Clinical Activity Index (CCECAI). Duodenal mucosal biopsy samples were endoscopically obtained for histopathologic examination by means of the World Small Animal Veterinary Association score. Double-labeled immunofluorescence was used to investigate colocalization of Ki-67 and CD3 in epithelium and lamina propria (LP) of villi and crypts. RESULTS: Dogs with CIE had significantly higher clinical score (median, 5.0; interquartile range [IQR], 3-7) compared to CO (all 0; P < .001). The Ki-67/CD3 double-positive cells were significantly increased in the LP of the crypt region of CIE dogs (0.63 cells/mm; IQR, 0-0.54) versus CO (0.08 cells/mm; IQR, 0-0.26; P = .044). A significant correlation was found between CCECAI and the Ki-67/CD3 ratio in the LP of the crypt region (r = 0.670; P = .012) in dogs with CIE. CONCLUSIONS AND CLINICAL IMPORTANCE: The Ki-67/CD3 ratio is upregulated in the LP crypt region of dogs with CIE and it correlates with clinical severity. Therefore, Ki-67/CD3 could be a useful tool for detection of CIE.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31825538/