Peer-reviewed veterinary case report
Laboratory Diagnosis and Quantification of African Swine Fever Virus Using Real-Time Polymerase Chain Reaction.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2022
- Authors:
- Netherton, Christopher L et al.
- Affiliation:
- The Pirbright Institute · United Kingdom
Abstract
Real-time polymerase chain reaction (PCR) for the detection of African swine fever virus (ASFV) is the tool of choice for the diagnostic laboratory and is a robust and easily scalable method for the researcher analyzing viral replication both in vitro and in vivo. In this chapter, we describe protocols for both quantitative real-time polymerase chain reactions (qPCR) and non-quantitative real-time polymerase chain reactions (real-time PCR) for the detection of African swine fever virus genome in a range of samples.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/35575888/