Peer-reviewed veterinary case report
How to test dogs for mange mites using centrifuge method
By Alvares, Felipe Boniedj Ventura et al.·Published in Experimental & applied acarology·2021·Departamento de Medicina Veteriná, Brazil·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Laboratory diagnosis of mange-causing mites in dogs using a modified centrifugation-flotation technique in sucrose solution.
- Species:
- dog
Plain-English summary
A group of dogs with skin problems, specifically scabies, were tested using two different methods to identify the mites causing the issue. The study compared a traditional microscope technique with a new method using a sucrose solution to see which was more effective. Both methods found similar rates of scabies, but the new method provided clearer results and took less time to read. This means that veterinarians can diagnose scabies more efficiently using the centrifugation-flotation technique, helping dogs get the treatment they need faster.
People also search for: dog skin problems scabies treatment · how to diagnose mange in dogs · effective treatments for dog mites
Abstract
Among the skin disorders of dogs, scabies is notable for its high occurrence rate and the need for veterinary interventions. There are two obstacles to making this diagnosis through direct investigation under a microscope (DIM): the continual need to make new slides when the results are negative and the long time needed for reading these slides. Thus, the objective of the present study was to compare efficacy between DIM and the technique of centrifugation-flotation in sucrose solution (CFSS) in samples from dogs in the semiarid region of the state of Paraíba, Brazil. Samples from 136 dogs were used, and three slides were made for each examination (DIM and CFSS). The readings were halted in cases of positivity. Positive samples were obtained from 56.6% of the dogs (77/136), of which 76.6% (59/77) were positive through both techniques, 13% (10/77) only through DIM and 10.4% (8/77) only through CFSS. The positivity rate did not differ statistically between the techniques. CFSS showed higher quality of readings, due to the considerably fewer artifacts on the slides, thereby optimizing the reading time. Sensitivity (85.6%), specificity (88.1%), accuracy (86.8%), positive predictive value (88.1%) and negative predictive value (85.1%) were obtained and the kappa coefficient (0.73) was considered substantial. It was concluded that CFSS showed high diagnostic capacity for scabies, similar to that of DIM, with optimized reading time, fewer artifacts and better display of mites.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/34557977/