Peer-reviewed veterinary case report
Large-Scale Isolation of Mouse Thymic Epithelial Cells.
- Year:
- 2023
- Authors:
- Ohigashi I et al.
- Affiliation:
- Institute of Advanced Medical Sciences · Japan
Abstract
The thymus is compartmentalized into the cortex and the medulla. Cortical and medullary thymic epithelial cells (TECs) characterize T cell-producing and T cell-selecting functions of cortical and medullary microenvironments in the thymus. Enzymatic digestion of the thymus and flow cytometric isolation of TECs and their subpopulations are useful for molecular and cellular characterization of TECs. However, the cellularity of cTECs and mTECs isolated from mouse thymus is limited. In this chapter, we describe the method for isolation of a large number of TECs using enlarged mouse thymus, which enables biochemical and proteomic analysis of TEC subpopulations.
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Search related cases →Original publication: https://europepmc.org/article/MED/36374458