Peer-reviewed veterinary case report
Lentivirus-mediated Wnt10b overexpression enhances fracture healing in a rat atrophic non-union model.
- Journal:
- Biotechnology letters
- Year:
- 2015
- Authors:
- Gao, Feng et al.
- Affiliation:
- Department of Orthopedic Surgery · China
- Species:
- rodent
Abstract
Lentivirus vectors encoding Wnt10b gene (LV-Wnt10b) or luciferase gene (LV-luc) were constructed to determine whether Wnt10b overexpression improved fracture healing in a rat atrophic non-union model. After fracture, rats were injected with LV-Wnt10b or LV-luc. Luciferase signals were clearly detected. At 2 and 4 weeks, LV-Wnt10b group had 107 and 98% more proliferating cell nuclear antigen (PCNA) positive cells, respectively, and promoted expression of bone morphogenetic protein-2 (BMP-2) in the callus compared with controls. LV-Wnt10b injection significantly increased bone mass density and bone mineral content: 46-84 and 96-193%, respectively, at the site of fracturein the LV-Wnt10b group compared with controls. At 8 weeks, fractured femora were healed in the LV-Wnt10b group compared with atrophic non-unions formed in controls. Thus, Wnt10b overexpression associated with lentiviral gene therapy is effective in healing atrophic non-unions in rats.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25348747/