Peer-reviewed veterinary case report
CO2 laser treatment effects on dog corneal cells seen under microscope
By Hoffman, Allison et al.·Published in Veterinary ophthalmology·2009·Eye Care For Animals, United States·View original on PubMed →
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Original publication title: Light and electron microscopic evaluation of canine corneal endothelium following CO2 photokeratotomy.
- Species:
- dog
Plain-English summary
A group of healthy dogs underwent a procedure called CO2 photokeratotomy to treat eye problems, and researchers wanted to see how this treatment affected the corneal endothelium (the inner layer of the cornea). After the procedure, they found that while the endothelial cells looked normal in some areas, six out of eight treated eyes showed signs of damage, and all treated eyes had increased corneal thickness. This thickening was linked to the level of damage observed. Overall, the study concluded that CO2 laser treatment does change the structure and thickness of the cornea in dogs.
People also search for: dog eye surgery effects · CO2 laser treatment for dogs · corneal thickness in dogs · dog eye problems after surgery
Abstract
OBJECTIVE: To determine using light and scanning electron microscopy if treatment with CO2 photokeratotomy alters the corneal endothelium in healthy dogs. PROCEDURE: Eight surgery laboratory dogs were determined to be free of ocular abnormalities. Under general anesthesia, the left eye of each dog was treated in a quadrant from 12 to 3 o'clock with the CO(2) laser in a defocused mode. The right eye served as a control. There were four treatment groups, each with 2 dogs: group 1 (2 W, 0.1 J/s, 0.8 mm tip), group 2 (3 W, 0.3 J/s, 0.8 mm tip), group 3 (2 W, 0.04 J/s NovaScan), group 4 (3 W, 0.06 J/s, NovaScan). The 0.8 mm tip delivered a power density of 382 W/cm(2) or 573 W/cm(2), at 2 or 3 watts respectively. The NovaScan handpiece delivered a power density of 30 W/cm(2) or 40 W/cm(2), at 2 or 3 Watts respectively. Following euthanasia, right and left corneas including a 2-mm scleral rim were harvested and fixed in commercial grade Karnovsky's fixative. One piece of cornea was processed routinely, embedded in Embed 812 resin, sectioned at 1 um, stained with toluidine blue and evaluated with the light microscope. A separate piece of each cornea was routinely processed and examined with a JEOL 6400 scanning electron microscope (SEM) at 20 KV. RESULTS: No changes in endothelial cell morphology were detected by light microscopy in the sections examined. SEM indicated normal endothelial cell morphology in control eyes with presumed artifactual changes at the corneal free margin (4/8 eyes). Multiple punctate to linear regions of endothelial cell destruction were observed in 6/8 laser-treated corneas. A significant increase in corneal thickness ranging from 1.90 to 37.28% was observed in all laser treated corneas. This increase in thickness correlated linearly with the degree of endothelial damage. Ultrastructural findings also correlated with postoperative clinical findings. CONCLUSION: CO2 laser photokeratotomy alters corneal endothelial cell morphology and thickness.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/19891649/