Peer-reviewed veterinary case report
Linear epitope recognition antibodies strongly respond to the C-terminal domain of HP-PRRSV GP5.
- Journal:
- Veterinary microbiology
- Year:
- 2014
- Authors:
- Wang, Xinglong et al.
- Affiliation:
- College of Veterinary Medicine · China
Abstract
A total of 155 peptides derived from the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) glycoprotein 5 (GP5) were printed on a chip to reveal the antigen reaction characteristics of the protein. The reactions of these peptides to HP-PRRSV-specific pig serum were scanned and quantified using fluorescence intensity via the PepSlide(®) Analyzer software. The intensity plots showed different reactions in the different sectors of GP5. The highest reaction intensity value reached 3894.5, with a peptide sequence of IVEKGGKVEVEGHLI. Seventeen peptides that showed relatively high reaction levels with HP-PRRSV-specific pig serum were selected as epitope candidates. Furthermore, the antigenic character was predicted using a software and was compared with the peptide scan results. In contrast to the software prediction, the HP-PRRSV-specific antibodies strongly responded to the C-terminal domain of GP5. The acquired data may be useful for understanding the antigenic characteristics of HP-PRRSV GP5.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25448446/