Peer-reviewed veterinary case report
Localization of infection-related epitopes on the non-structural protein 3ABC of foot-and-mouth disease virus and the application of tandem epitopes.
- Journal:
- Journal of virological methods
- Year:
- 2004
- Authors:
- Sun, Tao et al.
- Affiliation:
- Biotechnology Institute · China
- Species:
- rodent
Abstract
By means of overlapping peptides expressed in Escherichia coli in combination with Western-blotting, infection specific linear epitopes were identified on the non-structural protein 3ABC of FMDV. The epitopes reacted with sera from pigs or guinea pigs infected with different serotypes of FMDV, but not with sera from normal or vaccinated animals. A protein was constructed by tandem repeat of the epitope covering amino acid residues 141-190 on 3ABC. An ELISA based on the protein with tandem epitopes could be used as a diagnostic antigen for differentiating infected pigs from vaccinated ones.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/15158588/