Peer-reviewed veterinary case report
Locally administered lentivirus-mediated siRNA inhibits wear debris-induced inflammation in murine air pouch model.
- Journal:
- Biotechnology letters
- Year:
- 2008
- Authors:
- Peng, Xiaochun et al.
- Affiliation:
- Department of Orthopaedics · China
- Species:
- rodent
Abstract
Lentivirus(LV)-mediated small interfering RNA (siRNA) targeting tumor necrosis factor-alpha (TNF-alpha) was locally administered into the air pouch of mice to inhibit inflammation induced by titanium alloy particles. The lentiviral vector expressed green fluorescent protein (GFP) as a reporter gene. Down-regulation of TNF-alpha in pouch area was confirmed by real-time PCR and ELISA, resulting in significantly decreased local inflammatory responses (P < 0.01). This approach was proven safe by localized GFP fluorescence and invariant TNF-alpha expression in peripheral blood, liver, spleen, kidney, lung and brain of mouse. In conclusion, locally administered siRNA provides an effective and safe method for inhibiting particle-induced inflammation.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/18594768/