Peer-reviewed veterinary case report
Long-chain LPS-based enzyme-linked immunosorbent assay to detect swine herds infected byserotype 17.
- Journal:
- The Canadian veterinary journal = La revue veterinaire canadienne
- Year:
- 2021
- Authors:
- Gottschalk, Marcelo et al.
- Affiliation:
- Department of Infectious Disease · United Kingdom
Abstract
serotype 17, one of the two most recent serotypes described, has been isolated from diseased pigs in North America. Yet, no serological test for surveillance has been developed so far. An enzyme-linked immunosorbent assay (ELISA) using the long-chain lipopolysaccharide antigen (LC-LPS) of this serotype is described. As predicted by previous genetic data on the O-antigen locus, cross reactions were observed between this serotype and serotypes 3, 6, 8, and 15. Although animals infected by serotype 17 would be detected using the current serotype 3 LC-LPS ELISA, better results may be obtained when plates are coated with the antigen purified from the homologous serotype.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/33390602/