Long non-coding RNApromotes the proliferation of ovarian granulosa cells and steroid hormone production upregulation of.

Abstract

Granulosa cells (GCs) are essential for follicular development, and long non-coding RNAs (LncRNAs) are known to support the maintenance of this process and hormone synthesis in mammals. Nevertheless, the regulatory roles of these lncRNAs within sheep follicular GCs remain largely unexplored. This study delved into the influence of a, on the proliferation and steroid hormone synthesis of sheep ovarian GCs and the associated target genes. Cell Counting Kit-8 (CCK-8) gain-of-function experiments indicated that overexpression ofsignificantly boosted GCs proliferation, along with estrogen (E) and progesterone (P) levels. Further mechanistic scrutiny revealed thatis primarily localized within the cytoplasm of ovarian granulosa cells and engages in molecular interplay with. This interaction results in the upregulation ofprotein expression. Moreover, it was discerned that increasedlevels contribute to augmented proliferation of follicular granulosa cells and the secretion of Eand P. Experiments involving co-transfection elucidated that the concurrent overexpression ofandacted synergistically to potentiate these effects. These findings provide insights into the molecular underpinnings of fecundity in ovine species and may inform future strategies for enhancing reproductive outcomes.