Peer-reviewed veterinary case report
Loop-mediated isothermal amplification for rapid and convenient detection of Mycoplasma hyopneumoniae.
- Journal:
- World journal of microbiology & biotechnology
- Year:
- 2013
- Authors:
- Li, Jiahe et al.
- Affiliation:
- College of Veterinary Medicine · China
Plain-English summary
This study looked at a new method called loop-mediated isothermal amplification (LAMP) to quickly and easily detect a bacteria called Mycoplasma hyopneumoniae in the lungs of pigs. This bacteria can cause respiratory issues, and the LAMP method can find it using very small amounts of its genetic material in just 30 minutes, similar to another method called real-time PCR. The test is straightforward and inexpensive, as it can be done without complicated equipment and can visually show if the bacteria is present or not. It also effectively tells M. hyopneumoniae apart from other similar bacteria. Overall, the LAMP method was found to be a reliable way to diagnose this infection in nasal swabs and lung samples from pigs.
Abstract
Loop-mediated isothermal amplification (LAMP), a novel method of gene amplification, was employed in this study for detecting Mycoplasma hyopneumoniae in the respiratory tract or lungs of swine. The pathogen can be detected in LAMP reactions containing as few as 10 fg purified target DNA (10 copies of M. hyopneumoniae genome) within 30 min, which was comparable to real-time PCR. After 30-min reaction at 63 °C, the addition of a certain amount of dye (SYBR Green I and hydroxyl naphthol blue at a proper ratio) into the LAMP reaction system makes the results easily determined as positive or negative by visual inspection. In addition, the LAMP was able to distinguish between M. hyopneumoniae and other closely-related mycoplasma strains, indicating a high degree of specificity. The LAMP assay was more simple and cheap, since the reaction could be completed under isothermal conditions and less laboratorial infrastructure are required. And, it was proven reliable for M. hyopneumoniae diagnosis of nasal swab and lung samples from the field.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23184577/