<i>Gastrodia elata</i>-Derived Parishin Extracts Attenuate Aging by Modulating Oxidative Stress, Inflammation, Apoptosis, and Metabolism.

Abstract

This study investigates the anti-aging properties of <i>Gastrodia elata</i>-derived Parishin extracts (GEPE) by exploring its regulatory effects on oxidative stress, inflammatory responses, apoptosis, and metabolism. Network pharmacology identified 27 active components with parishins as core and 250 anti-aging intersection targets of <i>Gastrodia elata</i>. Molecular docking confirmed strong binding activity of parishins to key targets such as EGFR, AKT1, and ALB. In vitro experiments showed that the GEPE concentration dependently reduced the activity of senescence marker SA-β-gal in D-galactose-induced senescent mouse microglial cell (BV2). In vivo experiments showed that GEPE improved cognitive and muscle functions, elevated organ indices of liver, thymus, and spleen, alleviated hepatic lipid accumulation and neuronal damage, reduced oxidative stress, inhibited inflammatory responses and cell apoptosis, and corrected metabolic disorders in D-galactose-induced aging mice. These findings demonstrate that GEPE is associated with attenuated aging, potentially through coordinated modulation of oxidative stress, inflammation, apoptosis, and metabolic homeostasis, highlighting its potential as a candidate for anti-aging interventions.